TABLE 2.
Oligonucleotide primers for amplification and sequencing of loci used in MLST scheme
| Locus | Gene product | GenBank accession no. | Primer | Primer sequence (5′-3′) | Annealing temp (°C) |
|---|---|---|---|---|---|
| FKS | 1,3-Beta-glucan synthase | AF229171 | FKSF1 | GTCAAATGCCACAACAACAACCT | 55.0 |
| FKSR1 | AGCACTTCAGCAGCGTCTTCAG | ||||
| LEU2 | 3-Isopropylmalate dehydrogenase | U90626 | LEU2F1 | TTTCTTGTATCCTCCCATTGTTCA | 54.0 |
| LEU2R1 | ATAGGTAAAGGTGGGTTGTGTTGC | ||||
| NMT1 | Myristoyl-CoA, protein N-myristoyltransferasea | AF073886 | NMT1F1 | GCCGGTGTGGTGTTGCCTGCTC | 59.0 |
| NMT1R1 | CGTTACTGCGGTGCTCGGTGTCG | ||||
| TRP1 | Phosphoribosyl-anthranilate isomerase | U31471 | TRP1F1 | AATTGTTCCAGCGTTTTTGT | 50.0 |
| TRP1R1 | GACCAGTCCAGCTCTTTCAC | ||||
| UGP1 | UTP-glucose-1-phosphate uridylyltransferase | AB037186 | UGP1F1 | TTTCAACACCGACAAGGACACAGA | 57.0 |
| UGP1R1 | TCGGACTTCACTAGCAGCAAATCA | ||||
| URA3 | Orotidine-5′-phosphate decarboxylase | L13661 | URA3F1 | AGCGAATTGTTGAAGTTGGTTGA | 53.0 |
| URA3R1 | AATTCGGTTGTAAGATGATGTTGC |
a
CoA, coenzyme A.