Table 1.
Sequences and MALDI-TOF MS data of oligoribonucleotides used for the preparation of siRNAs. The position of mismatch for pairing with the wild type gene is indicated in bold and underlined.
| siRNA | Strand | Sequence | MW | MALDI-TOF MS |
|---|---|---|---|---|
| (calculated) | m/z | |||
| P1 | S: | 5′-GUGGUUGGUAAAGCCUCAGTT-3′ | 6724.1 | 6768.3 |
| As: | 3′-TTCACCAACCAUUUCGGAGUC-5′ | 6587.2 | 6592.1 | |
| P2 | S: | 5′-UGUGGUUGGUAAAGCCUCATT-3′ | 6685.1 | 6681.9 |
| As: | 3′-TTACACCAACCAUUUCGGAGU-5′ | 6611.2 | 6608.2 | |
| P3 | S: | 5′-UUGUGGUUGGUAAAGCCUCTT-3′ | 6662.1 | 6660.3 |
| As: | 3′-TTAACACCAACCAUUUCGGAG-5′ | 6634.2 | 6632.1 | |
| P4 | S: | 5′-GUUGUGGUUGGUAAAGCCUTT-3′ | 6702.1 | 6697.1 |
| As: | 3′-TTCAACACCAACCAUUUCGGA-5′ | 6594.2 | 6592.6 | |
| P5 | S: | 5′-UGUUGUGGUUGGUAAAGCCTT-3′ | 6702.1 | 6699.6 |
| As: | 3′-TTACAACACCAACCAUUUCGG-5′ | 6594.2 | 6590.5 | |
| P6 | S: | 5′-GUGUUGUGGUUGGUAAAGCTT-3′ | 6742.0 | 6736.5 |
| As: | 3′-TTCACAACACCAACCAUUUCG-5′ | 6554.3 | 6552.9 | |
| P7 | S: | 5′-AGUGUU CUGGUUGGUAAAGTT-3′ | 6766.0 | 6764.0 |
| As: | 3′-TTUCACAACACCAACCAUUUC-5′ | 6515.3 | 6514.1 | |
| P8 | S: | 5′-CAGUGUUGUGGUUGGUAAATT-3′ | 6726.1 | 6723.5 |
| As: | 3′-TTGUCACAACACCAACCAUUU-5′ | 6555.3 | 6552.7 | |
| P9 | S: | 5′-ACAGUGUUGUGGUUGGUAATT-3′ | 6726.1 | 6725.1 |
| As: | 3′-TTUGUCACAACACCAACCAUU-5′ | 6555.3 | 6553.2 | |
| P10 | S: | 5′-UACAGUGUUGUGGUUGGUATT-3′ | 6703.0 | 6705.5 |
| As: | 3′-TTAUGUCACAACACCAACCAU-5′ | 6578.3 | 6578.6 | |
| P11 | S: | 5′-CUACAGUGUUGUGGUUGGUTT-3′ | 6679.0 | 6720.2 |
| As: | 3′-TTGAUGUCACAACACCAACCA-5′ | 6617.2 | 6615.5 | |
| P12 | S: | 5′-UCUACAGUGUUGUGGUUGGTT-3′ | 6679.0 | 6679.4 |
| As: | 3′-TTAGAUGUCACAACACCAACC-5′ | 6617.2 | 6617.1 | |
| P13 | S: | 5′-UUCUACAGUGUUGUGGUUGTT-3′ | 6640.1 | 6640.2 |
| As: | 3′-TTAAGAUGUCACAACACCAAC-5′ | 6641.3 | 6664.1 | |
| P14 | S: | 5′-UUUCUACAGUGUUGUGGUUTT-3′ | 6601.1 | 6601.1 |
| As: | 3′-TTAAAGAUGUCACAACACCAA-5′ | 6400.0 | 6701.1 | |
| P15 | S: | 5′-UUUUCUACAGUGUUGUGGUTT-3′ | 6601.1 | 6620.4 |
| As: | 3′-TTAAAAGAUGUCACAACACCA-5′ | 6665.3 | 6686.7 | |
| P16 | S: | 5′-AUUUUCUACAGUGUUGUGGTT-3′ | 6624.1 | 6669.3 |
| As: | 3′-TTUAAAAGAUGUCACAACACC-5′ | 6642.3 | 6640.1 | |
| P17 | S: | 5′-CAUUUUCUACAGUGUUGUGTT-3′ | 6584.1 | 6583.3 |
| As: | 3′-TTGUAAAAGAUGUCACAACAC-5′ | 6682.2 | 6679.5 | |
| P18 | S: | 5′-UCAUUUUCUACAGUGUUGUTT-3′ | 6545.2 | 6583.8 |
| As: | 3′-TTAGUAAAAGAUGUCACAACA-5′ | 6706.2 | 6705.0 | |
| P19 | S: | 5′-UUCAUUUUCUACAGUGUUGTT-3′ | 6545.2 | 6543.6 |
| As: | 3′-TTAAGUAAAAGAUGUCACAAC-5′ | 6706.2 | 6704.4 |