Table 2.

Differences between the free Gibbs energy of 5′-end and 3′-end of the duplex (resp. to the polarity of the antisense strand), calculated independently for three and for four consecutive base pairs shown for each duplex (ΔΔG_37°C = ΔG_37°C(5′-end) − ΔG_37°C  (3′-end)). The values of ΔG_37°C were calculated according to [60].

siRNA	Sequence	ΔΔG37°C (kcal/mol)	ΔΔG37°C (kcal/mol)
		(for 3 bp)	(for 4 bp)
P1	5′-GUGG—UCAGTT-3′	1	1.6
	3′-TTCACC—AGUC-5′
P2	5′-UGUG—CUCATT-3′	−0.2	−0.1
	3′-TTACAC—GAGU-5′
P3	5′-UUGU—CCUCTT-3′	−1.9	−2.7
	3′-TTAACA—GGAG-5′
P4	5′-GUUG—GCCUTT-3′	−0.5	−1.8
	3′-TTCAAC—CGGA-5′
P5	5′-UGUU—AGCCTT-3′	−3	−3.8
	3′-TTACAA—UCGG-5′
P6	5′-GUGU—AAGCTT-3′	−1.2	0
	3′-TTCACA—UUCG-5′
P7	5′-AGUG—AAAGTT-3′	0.7	1.6
	3′-TTUCAC—UUUC-5′
P8	5′-CAGU—UAAATT-3′	1.7	2.7
	3′-TTGUCA—AUUU-5′
P9	5′-ACAG—GUAATT-3′	1.4	1
	3′-TTUGUC—CAUU-5′
P10	5′-UACA—GGUATT-3′	0	−1.1
	3′-TTAUGU—CCAU-5′
P11	5′-CUAC—UGGUTT-3′	−1.7	−1.4
	3′-TTGAUG—ACCA-5′
P12	5′-UCUA—UUGGTT-3′	−0.7	−0.5
	3′-TTAGAU—AACC-5′
P13	5′-UUCU—GUUGTT-3′	0.5	0.1
	3′-TTAAGA—CAAC-5′
P14	5′-UUUC—GGUUTT-3′	−0.7	−1.3
	3′-TTAAAG—CCAA-5′
P15	5′-UUUU—UGGUTT-3′	−2.7	−3.6
	3′-TTAAAA—ACCA-5′
P16	5′-AUUU—GUGGTT-3′	−3.4	−4.6
	3′-TTUAAA—CACC-5′
P17	5′-CAUU—UGUGTT-3′	−1.2	−2.1
	3′-TTGUAA—ACAC-5′
P18	5′-UCAU—UUGUTT-3′	0.7	0.7
	3′-TTAGUA—AACA-5′
P19	5′-UUCA—GUUGTT-3′	0.5	0.2
	3′-TTAAGU—CAAC-5′