TABLE 1.

Comparison of rates of detection of EV in CSF specimens of patients with aseptic meningitis by viral cell culture, in-house reference RT-PCR, and the new Penter RT-PCR assay

Cell culture result	Total no. of specimens	No. of specimens positive by:		No. of specimens negative by:
		Penter RT-PCRa	In-house RT-PCRb	Penter RT-PCR	In-house RT-PCR
Positive	28	28	28	0	0
Negative	26	15	13	11	13

^aPenter RT-PCR, RT-PCR amplification of EV genomes by using the principle of stair primers (5).

^bIn-house reference RT-PCR assay, seminested RT-PCR amplification of EV genomes (1).