Fig. 3.

Whole-cell extracts of C. burnetii RSA 439 phase II grown in mouse L929 cell culture and separated by 2D gel electrophoresis on pH 5–8 IEF gradients and 10.5–14 % acrylamide SDS-PAGE gels. C. burnetii seroreactive proteins are listed in Table 3. Molecular mass standards (kDa) are indicated on the left. (a) Sypro Ruby-stained gel showing seroreactive spots. Immune sera-reactive spots are labelled with numbers alone. Sham sera-reactive spots are labelled with numbers and an N prefix. (b) Immunoblot probed with pooled immune guinea pig serum. (c) Immunoblot probed with pooled serum from sham-immunized guinea pigs.