Table 1.
Phase I (egg) C. burnetii Nine Mile RSA 493 proteins identified by LC-MS/MS in seroreactive spots separated on pH 3–10 gradient gels
| Spot no.* | Locus tag† | Putative protein‡ | Protein score§ | Sequence coverage | No. of unique peptides with P value <0.05|| | Predicted Mr (kDa)/pI |
|---|---|---|---|---|---|---|
| 13 | CBU1706 | Thioredoxin peroxidase, AhpC/Tsa family | 71 | 20 % | 2 | 21.8/5.08 |
| 14 | CBU1290 | DnaK chaperone | 116 | 13 % | 1 | 70.7/5.14 |
| 15 | CBU0495 | 3-Oxoacyl-(acyl-carrier-protein) reductase FabG | 112 | 14 % | 2 | 26.3/7.63 |
| CBU1910 | com1 | 65 | 14 % | 1 | 27.6/9.08 | |
| 17 | CBU1910 | com1 | 55 | 14 % | 1 | 27.6/9.08 |
| 18 | CBU1910 | com1 | 165 | 23 % | 3 | 27.6/9.08 |
| CBU0780 | DNA-binding response regulators GacA | 54 | 6 % | 1 | 24.2/9.10 | |
| CBU0955 | 6 % | 1 | 24.1/9.52 | |||
| 27 | CBU0229 | Rp1L ribosomal protein L7/L12 | 84 | 38 % | 1 | 13.2/4.71 |
| N4 | CBU0263 | RpoA DNA-directed RNA polymerase, alpha | 205 | 12 % | 4 | 35.5/5.61 |
*Corresponds to spot shown in Fig. 1.
†TIGR annotation.
‡Described in Beare et al. (2009), Supplemental file 1, Supplemental Table S5.
§Mascot protein scores (standard scoring) are derived from combined peptide ion scores as a non-probabilistic basis for ranking protein hits.
||The number of unique peptides identified with ion scores >24 that indicate identity or extensive homology (P<0.05). Ion score is −10×log(P), where P is the probability that the match is a random event.