FIG. 2.
PCR results for the presence of IS1655 in the orf2 gene of the N. meningitidis strains examined. Lane M, molecular size markers, 100-bp DNA ladder (New England Biolabs). Lanes 1 and 2 show the presence of the amplicon of about 1,460 bp in one serogroup C strain representative of the ST11/ET-37 complex and in the B:2b:P1.5 switched ST1860/ET-37 strain, respectively. Lane 3 shows a shorter amplicon of about 390 bp in a strain representative of ST8/A4, which lacks the IS1655. Lane 4 shows the absence of the amplicon in a strain representative of ST44/lineage III.
