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RT-PCR analysis of six VRE isolates and a plasmid-free enterococcal strain (BM4105-RF). The VRE isolates tested were C29113, S34, S177, SL242, SL266 and SL745. (a) RT-PCR with primers complementary to axe–txe (product size=447 bp). (b) Controls for DNA contamination, in which reverse transcriptase was omitted from the reaction mix. (c) RT-PCR with primers complementary to the enterococci tuf gene (product size=112 bp). Lane M, DNA molecular mass markers.
