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Protein synthesis following IPTG induction of Txe–His6, Axe–His6 and empty pET21a vector. Incorporation of [3H]leucine immediately before and following addition of 0.05 mM IPTG to E. coli Rosetta BL21(DE3) carrying pET21a, pET21a-txe-His6 or pET21a-axe-His6. Results are the mean of three independent experiments. Error bars, sd; P, statistical significance between designated groups.
