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. 2011 Feb;157(Pt 2):387–397. doi: 10.1099/mic.0.045492-0

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Fig. 5. — Effect of Txe–His₆, Axe–His₆ and His₆–Axe on cell-free protein synthesis. (a) Western blot with HisProbe–HRP of cell-free protein synthesis reactions with no DNA added (lane 1), pET28a-relB-His₆ DNA added (2), pET28a-relB-His₆ and pET21a-txe-His₆ DNA added (3), and pET28a-relB-His₆ and pET21a-axe-His₆ DNA added (4). (b) Western blot with HisProbe-HRP of cell-free protein synthesis reactions with no DNA added (lane 1), pRSF1b-His₆-lpp DNA added (2), pRSF1b-His₆-lpp and pET21a-txe-His₆ DNA added (3), and pRSF1b-His₆-lpp and pACYCDuet1-His₆-axe DNA added (4). M, Marker lane.