Table 1.
ORFs of plasmid pS177
| ORF* | Gene | Nucleotide position | Protein length (aa) | Database match (GenBank accession no.) | Amino acid identity (%) | |
|---|---|---|---|---|---|---|
| 5′ | 3′ | |||||
| 1c | prgN | 1 | 291 | 97 | pRUM PrgN (AAO52827) | 100 |
| 2c | orf2 | 603 | 980 | 126 | pRUM conserved hypothetical protein, Ent. faecium (AAO52828) | 100 |
| 3c | uvrA | 946 | 2271 | 442 | pRUM UvrA, structural gene for UV resistance (AAO52829) | 100 |
| 4 | sin | 2784 | 3335 | 184 | pRUM Sin recombinase (AAO52830) | 100 |
| 5c | orf5 | 3500 | 4504 | 335 | pRUM hypothetical protein (AAO52831) | 100 |
| 6c | txe | 4963 | 5220 | 86 | pRUM Txe, toxin component of TA system (AAO52832) | 100 |
| 7c | axe | 5213 | 5482 | 90 | pRUM Axe, antitoxin component of TA system (AAO52833) | 100 |
| 8 | orf8 | 5824 | 6126 | 101 | pRUM conserved hypothetical protein (AAO52834) | 100 |
| 9 | orf9 | 6169 | 6387 | 73 | pRUM conserved hypothetical protein (AAO52835) | 100 |
| 10 | tnp (IS6) | 6559 | 7245 | 229 | pRUM IS6 transposase (AAO52848) | 100 |
| 11c | corA | 7536 | 8924 | 463 | Ent. faecium magnesium/nickel/cobalt transporter CorA (ZP_05660433) | 100 |
| 12c | int | 8989 | 9942 | 318 | Ent. faecium integrase, catalytic region (ZP_00603158) | 100 |
| 13c | orf13 | 10 026 | 10 156 | 43 | p5753cA hypothetical protein (ADA62235) | 100 |
| 14 | res | 10 585 | 11 148 | 188 | p5753cA resolvase, N-terminal domain (ADA62236) | 100 |
| 15 | tnp (IS1678) | 11 336 | 12 655 | 440 | Ent. faecium transposase, IS1678 (AAW32124) | 99 |
| 16c | relEEf | 13 111 | 13 398 | 96 | Lactobacillus antri RelE-like toxin component of TA system (ZP_05745775) | 53 |
| 17c | relBEf | 13 388 | 13 717 | 110 | Ent. faecalis Abr-family antitoxin component of TA system (ZP_06623423) | 79 |
| 18 | cadE | 14 022 | 14 351 | 110 | p5753cA cadmium efflux system accessory protein (ADA62239) | 100 |
| 19c | vanZ | 14 638 | 15 123 | 162 | Tn1546 VanZ, teicoplanin resistance protein (AAA65959) | 100 |
| 20c | vanY | 15 276 | 16 187 | 304 | Tn1546 VanY, truncated carboxypeptidase (AAA65958) | 99 |
| 21c | vanX | 16 615 | 17 223 | 203 | Tn1546 VanX, d-Ala–d-Ala dipeptidase (AAA65957) | 99 |
| 22c | vanA | 17 229 | 18 260 | 344 | Tn1546 VanA, d-Ala–d-Lac ligase (AAA65956) | 99 |
| 23c | vanH | 18 253 | 19 221 | 323 | Tn1546 VanH, pyruvate dehydrogenase (AAA65955) | 100 |
| 24 | tnp (IS1251) | 19 553 | 20 845 | 431 | Ent. faecium IS1251-like transposase (AAF73111) | 100 |
| 25c | vanS | 20 943 | 22 097 | 385 | Tn1546 VanS, truncated histidine kinase (AAA65954) | 100 |
| 26c | vanR | 22 075 | 22 770 | 232 | Tn1546 VanR, regulator of two-component regulatory system (AAA65953) | 100 |
| 27c | res | 22 984 | 23 559 | 192 | Tn1546 resolvase (AAA65952) | 100 |
| 28 | tnp (Tn1546) | 23 705 | 25 856 | 717 | Tn1546 transposase (AAA65951) | 100 |
| 29 | tnp (IS6) | 26 661 | 27 347 | 229 | pRUM IS6 transposase (AAO52854) | 100 |
| 30c | aph-3 | 28 195 | 28 980 | 262 | S. intermedius Aph-3, aminoglycoside phosphotransferase (AAG42234) | 100 |
| 31c | sat4 | 29 082 | 29 612 | 177 | S. intermedius Sat4, streptothricin acetyltransferase (AAG42233) | 100 |
| 32c | aadE | 29 621 | 30 529 | 303 | S. intermedius AadE, streptomycin adenyltransferase (AAG42232) | 100 |
| 33c | MTase | 30 562 | 31 296 | 245 | Ent. faecium methyltransferase (ZP_00603123) | 100 |
| 34c | dnaP | 31 277 | 32 146 | 290 | Ent. faecium DNA polymerase, beta-like region (ZP_00603124) | 100 |
| 35c | tnp (IS1182) | 32 521 | 33 195 | 225 | S. intermedius transposase, IS1182 (AAG42229) | 99 |
| 36c | orf36 | 33 652 | 33 783 | 44 | S. intermedius orf3 (AAG42228) | 100 |
| 37c | ermB | 33 788 | 34 525 | 246 | S. intermedius ErmB, erythromycin resistance methylase (AAG42227) | 100 |
| 38 | tnp (IS6) | 34 873 | 35 559 | 229 | pRUM IS6 transposase (AAO52854) | 100 |
| 39c | repA | 36 175 | 37 215 | 347 | pRUM putative RepA replication protein (AAO52855) | 100 |
| 40c | orf40 | 37 564 | 37 890 | 109 | pRUM hypothetical protein (AAO52856) | 100 |
| 41c | soj | 37 877 | 38 680 | 268 | pRUM Soj partitioning protein (AAO52857) | 100 |
*ORFs predicted to be transcribed on the complementary strand are denoted with ‘c’.