Fig. 1.

TagA is secreted by V. cholerae in a ToxR-dependent manner. (a) V. cholerae O395 and the isogenic ToxR null mutant strain JJM43 were grown under ToxR-inducing conditions until late-exponential phase. Cells were pelleted, supernatants precipitated with TCA, and samples were analysed by SDS-PAGE followed by immunoblotting with a polyclonal antibody raised to an N-terminal peptide of TagA. (b) Experiments were performed as described for (a), comparing V. cholerae O395 wt with the ΔtagA strain.