TABLE 1.
PCR primers for detection of MBL and integrase genes
| Gene | Primer name (sequence) | Expected size of amplicon (bp) | Reference or source |
|---|---|---|---|
| MBL genes | |||
| blaIMP-1 | F1 (5′-ACC GCA GCA GAG TCT TTG CC-3′) | 587 | This study |
| R1 (5′-ACA ACC AGT TTT GCC TTA CC-3′) | |||
| blaIMP-2 | F2 (5′-GTT TTA TGT GTA TGC TTC C-3′) | 678 | This study |
| R2 (5′-AGC CTG TTC CCA TGT AC-3′) | |||
| blaVIM-1 | F3 (5′-AGT GGT GAG TAT CCG ACA G-3′) | 261 | 24 |
| R3 (5′-ATG AAA GTG CGT GGA GAC-3′) | |||
| blaVIM-2 | F4 (5′-ATG TTC AAA CTT TTG AGT AAG-3′) | 801 | 17a |
| R4 (5′-CTA CTC AAC GAC TGA GCG-3′) | |||
| blaSPM-1 | F5 (5′-GCG TTT TGT TTG TTG CTC-3′) | 786 | This study |
| R5 (5′-TTG GGG ATG TGA GAC TAC-3′) | |||
| Integrase genes | |||
| intI1 | F6 (5′-GCA TCC TCG GTT TTC TGG-3′) | 457 | This study |
| R6 (5′-GGT GTG GCG GGC TTC GTG-3′) | |||
| intI2 | F7 (5′-CAC GGA TAT GCG ACA AAA AGG T-3′) | 789 | This study |
| R7 (5′-GTA GCA AAC GAG TGA CGA AAT G-3′) | |||
| intI3 | F8 (5′-ATC TGC CAA ACC TGA CTG-3′) | 922 | This study |
| R8 (5′-CGA ATG CCC CAA CAA CTC-3′) | |||
| Coamplification of the intI3-blaIMP-1 region | F9 (5′-GGT CTT GTA GGC TGT AAT TG-3′) | 609 | This study |
| R9 (5′-TTG TGG CTT GGA ACC TTT AC-3′) |
a
Since the nucleotide sequence appearing in reference 17 has a typographical error, the accurate sequence is shown in this table.