TABLE 1.
General primer sets
| Primer name | ORFa of viral genome | Primer sequence (5′-3′) | Primer locationb | PCR product size (bp) | Reference(s) |
|---|---|---|---|---|---|
| L1C1 | L1 | CGTAAACGTTTTCCCTATTTTTTT | 5609-5632 | 243-262 | 9, 37 |
| L1C2 | TACCCTAAATACTCTGTATTG | 5841-5861 | |||
| (L1C2M) | (TACCCTAAATACCCTATATTG) | (5841-5861) | |||
| GP5+ | L1 | TTTGTTACTGTGGTAGATAC | 6624-6643 | 139-149 | 25 |
| GP6− | GAAAAATAAACTGTAAATCA | 6746-6765 | |||
| L1S-L | L1 | TTTAATAAACCATATTGGTTACA | 6555-6577 | 89 | —c |
| L1S-R | GTATCCACAACAGTAACAAA | 6624-6643 | |||
| PU-1M | E6/E7 | TGTCAAAAACCGTTGTGTCC | 419-438 | 205-271 | 38 |
| (pU-1M-L) | (TGTCAAAAACCGTTGTGTCCAGAAGAAAA) | (419-447) | |||
| pU-2R | GAGCTGTCGCTTAATTGCTC | 637-656 | |||
| (pU-2R-N) | (TCTGAGTCGCTTAATTGCTC) | (637-656) | |||
| E6-L | E6 | ACCGAAAACGGTTCATATAAA | 50-70 | 192-212 | —c |
| E6-R | AAATGCAAATTCATATACCTC | 224-244 |
a
ORF, open reading frame.
b
Primer location refers to the sequence of HPV type 16 (GenBank accession no. K02718).
c
—, new primer design.