TABLE 2.
Comparison of mismatches between general primer sets and HPVs DNAa
| HPV type (associated riskb) | GenBank accession No. | No. of nucleotide mismatches
|
||||||
|---|---|---|---|---|---|---|---|---|
| GP5+/GP6− | L1C1/L1C2 | L1C1/L1C2M | pU-1M/pU-2R | pU-1M-L/pU-2R-N | L1S-L/L1S-R | E6-L/E6-R | ||
| 16 (HR) | K02718 | 2/0 | 2/3 | 2/3 | 3/2 | 4/7 | 2/1 | 3/2 |
| 18 (HR) | X05015 | 0/1 | 1/2 | 1/2 | 4/5 | 5/0 | 0/2 | 2/1 |
| 31 (IR) | J04353 | 0/2 | 4/3 | 4/1 | 1/2 | 1/7 | 2/2 | 3/3 |
| 33 (IR) | A12360 | 0/2 | 1/4 | 1/2 | 2/1 | 4/6 | 2/2 | 2/1 |
| 35 (IR) | M74117 | 2/0 | 5/0 | 5/3 | 1/3 | 3/8 | 1/2 | 3/3 |
| 39 (IR) | M62849 | 4/2 | 1/5 | 1/3 | 3/8 | 4/3 | 3/2 | 3/0 |
| 45 (IR) | X74479 | 3/0 | 1/5 | 1/3 | 6/6 | 7/1 | 1/2 | 2/2 |
| 51 (IR) | M62877 | 6/3 | 2/1 | 2/1 | 6/10 | 7/5 | 4/5 | 1/5 |
| 52 (IR) | X74481 | 4/1 | 1/4 | 1/4 | 4/2 | 5/7 | 2/2 | 3/5 |
| 53 (IR) | X74482 | 3/4 | 2/5 | 1/3 | 4/9 | 6/4 | 3/1 | 1/3 |
| 56 (IR) | X74483 | 1/3 | 1/2 | 1/0 | 5/10 | 7/5 | 2/2 | 1/4 |
| 58 (IR) | D90400 | 2/2 | 1/5 | 1/3 | 2/2 | 3/7 | 3/3 | 3/2 |
| 59 (IR) | X77858 | 4/2 | 1/1 | 1/3 | 5/7 | 8/4 | 2/4 | 1/2 |
| 66 (IR) | U31794 | 2/4 | 1/3 | 1/3 | 7/10 | 9/5 | 2/0 | 0/5 |
| 68 (IR) | M73258 | 3/3 | 3/4 | 3/4 | 5/8 | 8/3 | 5/1 | 2/1 |
| 70 (IR) | U21941 | 3/2 | 1/3 | 1/1 | 4/9 | 5/4 | 3/3 | 2/0 |
| 82 (IR) | AF293961 | 6/2 | 2/4 | 2/2 | 4/10 | 8/5 | 2/5 | 1/5 |
| 6b (LR) | X00203 | 0/1 | 3/3 | 3/3 | 4/3 | 5/8 | 2/2 | 6/6 |
| 11 (LR) | M14119 | 0/0 | 3/3 | 3/3 | 4/3 | 6/8 | 2/2 | 6/5 |
| 26 (LR) | X74472 | 5/3 | 3/4 | 3/4 | 5/11 | 5/6 | 2/6 | 2/4 |
| 34 (LR) | X74476 | 3/5 | 3/3 | 3/3 | 3/10 | 3/5 | 3/3 | 4/3 |
| 40 (LR) | X74478 | 3/1 | 9/1 | 9/1 | 5/9 | 8/4 | 5/3 | 2/9 |
| 42 (LR) | M73236 | 3/4 | 4/3 | 4/5 | 5/11 | 9/6 | 0/4 | 3/10 |
| 43 (LR) | U12504 | —/—c | —/— | —/— | 7/— | 9/— | —/3 | 3/8 |
| 44 (LR) | U31788 | 1/3 | 4/6 | 4/4 | 5/9 | 6/4 | 4/1 | 5/3 |
| 54 (LR) | NC_001676 | 4/1 | 3/4 | 2/2 | 6/8 | 9/4 | 2/4 | 2/6 |
| 73 (LR) | X94165 | 3/1 | 5/4 | 5/4 | 3/11 | 6/6 | 2/3 | 2/1 |
a
The published primer sequence was compared to HPV DNA sequences obtained from GenBank (http://www.ncbi.nlm.nih.gov) and the HPV sequence database at Los Alamos National Laboratory (http://hpv-web.lanl.gov).
b
HR, high risk; IR, intermediate risk; LR, low risk.
c
—, for HPV type 43, only a partial sequence is available from the indicated sources.