TABLE 4.

Five different gradient conditions for HPV genotyping^a

Step	General			Group I			Group II			Group III			Group IV
	Time (min)	% A	% B	Time (min)	% A	% B	Time (min)	% A	% B	Time (min)	% A	% B	Time (min)	% A	% B
Loading	0.0	49	51	0.0	49	51	0.0	49	51	0.0	48	52	0.0	47	53
Start gradient	0.5	48	52	0.5	48	52	0.5	48	52	0.5	47	53	0.5	46	54
Stop gradient	7.3	45	55	5.0	47	53	5.0	47	53	5.0	46	54	9.0	45	55
Start clean	7.4	0	100	5.1	0	100	5.1	0	100	5.1	0	100	9.1	0	100
Stop clean	7.9	0	100	5.6	0	100	5.6	0	100	5.6	0	100	9.5	0	100
Start equilibrate	8.0	49	51	5.7	49	51	5.7	49	51	5.7	48	52	9.6	47	55
Stop equilibrate	11.0	49	51	6.8	49	51	6.8	49	51	6.8	48	52	12.6	47	55

^aSee Fig. 3 for the HPV composition of each group. A and B refer to buffers A and B, respectively, as defined in the text.