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. 2011 Mar 19;20(11):2251–2262. doi: 10.1093/hmg/ddr115

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© The Author 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Schematic representation of the expression cassettes containing EPO and EPO-D. Each expression construct has been cloned between the AAV2-inverted terminal repeats using the pAAV2.1 plasmid for AAV2/1 vector production. EPO, S100E, NP1 and NP2 CDSs are all of murine origin. The amino acidic region relative to the murine EPO protein is shown. CMV, cytomegalovirus promoter; EPO, erythropoietin; EPO SP, EPO signal peptide; polyA, polyadenilation signal sequence; S100E, EPO carrying the S to E mutation at amino acidic position 100 of the mature EPO (corresponding to amino acidic position 126 from the ATG start codon).