TABLE 1.
Reproducibility of the BTUB FRET PCR assay
| Technician | Standard (known concn) | Mean calculated concn (SD)a,b | Mean Ct (SD)a,c |
|---|---|---|---|
| 1 | 1 (84) | 88.1 (21.4) | 30.2 (0.40) |
| 2 (42) | 38.8 (5.65) | 31.5 (0.23) | |
| 3 (21) | 19.4 (3.81) | 32.6 (0.35) | |
| 4 (10.5) | 12.2 (1.50) | 33.3 (0.21) | |
| 5 (5.25) | 6.50 (3.30) | 34.5 (0.78) | |
| 6 (1.06) | 0.84 (0.03) | 37.6 (0.05) | |
| 2 | 1 (84) | 91.1 (21.6) | 31.0 (0.45) |
| 2 (42) | 45.6 (10.5) | 32.4 (0.44) | |
| 3 (21) | 22.3 (3.80) | 33.5 (0.33) | |
| 4 (10.5) | 10.6 (2.47) | 34.7 (0.40) | |
| 5 (5.25) | 4.48 (0.95) | 36.4 (0.39) | |
| 6 (1.06) | 1.62 (1.10) | 38.4 (1.30) | |
| Pooled means | 1 (84) | 89.6 (21.5) | 30.6 (0.43) |
| 2 (42) | 40.7 (8.10) | 31.9 (0.33) | |
| 3 (21) | 20.8 (3.80) | 33.1 (0.34) | |
| 4 (10.5) | 11.4 (1.90) | 34.1 (0.30) | |
| 5 (5.25) | 5.40 (2.10) | 35.5 (0.60) | |
| 6 (1.06) | 1.23 (0.60) | 38.0 (0.70) |
a
All values are means based on five replicates analyzed for each technician and 10 replicates analyzed for the pooled means.
b
T. vaginalis organisms per PCR, generated by the LightCycler utilizing Ct and the known concentration of the standards.
c
Ct was generated based on the cycle number where the LightCycler software interpreted a logarithmic increase in fluorescence of the given sample.