TABLE 2.
Initial and resolved sensitivity and specificity of the BTUB FRET assay
| Data type | PCR | Sensitivity (%) | Specificity | PPV | NPV |
|---|---|---|---|---|---|
| Initiala | TETR | 100 (20/20) | 100 (233/233) | 100 (20/20) | 100 (233/233) |
| BTUB FRET | 75.0 (15/20) | 98.3 (229/233) | 78.9 (15/19) | 97.8 (229/234) | |
| Revolvedb | TETR | 81 (17/21) | 98.7 (232/235) | 85.0 (17/20) | 98.3 (232/236) |
| BTUB FRET | 90.4 (19/21) | 100 (232/232) | 100 (19/19) | 99.1 (232/234) |
a
Sensitivity and specificity of BTUB FRET compared to TETR PCR.
b
Sensitivity and specificity where infection status is determined by two positive PCR results, BTUB FRET and TVK3/4 PCR (14).