TABLE 2.
Quantification of CYP27A1 in human RPE
| Donor (pmol/mg tissue protein)a |
||||
|---|---|---|---|---|
| CYP27A1 peptide | #12 | #13 | #17 | #20 |
| RPE | ||||
| LYPVVPTNSR | 1.45 ± 0.12 | 2.06 ± 0.12 | 1.11 ± 0.33 | 1.61 ± 0.23 |
| VVLAPETGELK | 1.30 ± 0.16 | 1.90 ± 0.23 | 1.15 ± 0.11 | 1.39 ± 0.10 |
| EIEVDGFLFPK | 1.65 ± 0.32 | 2.08 ± 0.20 | 1.26 ± 0.12 | 1.72 ± 0.05 |
| Consensus | 1.46 ± 0.24 | 2.01 ± 0.18 | 1.17 ± 0.20 | 1.57 ± 0.19 |
| Neural retinab | ||||
| Consensus | 0.46 ± 0.04 | 0.51 ± 0.05 | 0.57 ± 0.05 | 0.53 ± 0.06 |
a
The concentration was calculated for three experimental replicates by monitoring three transitions per individual peptide and presented as mean ± SD. For the consensus, the data for three peptides from CYP27A1 were combined and presented as mean ± SD. The monitored transitions are the same as in Ref. 24.
b
Taken from Ref. 24.