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. 2011 Jun;52(6):1128–1138. doi: 10.1194/jlr.M014415

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Copyright ©2011 by the American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 9. — Elovl4 Tg mRNA is not expressed in the retina. Nuclease protection assay for wt Elovl4, Stgd3, and Tg mRNAs. A: The skin of three-week-old Tg mice expresses Tg mRNA in addition to wt Elovl4 mRNA and/or Stgd3 mRNA. The eyecups of three-week-old Tg^A+C/homozygous Stgd3 mice (Tg St/St) express Stgd3 mRNA but neither wt Elovl4 mRNA nor Tg mRNA. In this assay, wt Elovl4 mRNA protects a 342-N fragment of the 414-N riboprobe, Tg mRNA protects a 287-N fragment, and Stgd3 mRNA protects two fragments of 230 and 107 Ns, as shown in schematic form in B.