TABLE 1.

Accuracy and precision of D-4F plasma measurements

Peptide Added (ng/ml)	1,000	200	40	10	1
Values obtained from quadruplicates (ng/ml)	925	206	41.8	9.31	1.32
	965	192	34.5	8.46	1.17
	961	187	35.4	7.24	0.931
	1090	185	35.0	6.75	1.03
Mean ± SD	985.3 ± 72	192.5 ± 9.47	36.68 ± 3.43	7.94 ± 1.16	1.11 ± 0.169
95% CI	(870.5–1100)	(177.4–207.6)	(31.21–42.14)	(6.09–9.79)	(0.84–1.38)
CV%	7.3%	4.9%	9.4%	14.6%	15.2%

A plasma sample from an apoE null mouse that had not received peptide was spiked with D-4F to give a plasma concentration of 1,000, 200, 40, 10, or 1 ng/ml. The plasma was extracted and the D-4F concentration was determined in quadruplicate as described in Materials and Methods.