TABLE 3.

Normalized levels of DHCEO (ng/mg protein) formed from 5 μM 1 (epoxide) or 2 (triol) in the presence of different cell lines over 24 h

	HF-control		HF-SLOS		Neuro2a-control
Substrate compound	1	2	1	2	1	2
DHCEO in Cells (ng/mg)	238 ± 19	717 ± 31	204 ± 26	808 ± 25	2630 ± 184	2242 ± 164
DHCEO in medium (ng/mg)	414 ± 47	1911 ± 291	324 ± 42	1058 ± 46	1240 ± 56	1470 ± 36
Total DHCEO (ng/mg)	653 ± 33	2629 ± 316	527 ± 68a	1865 ± 69a	3870 ± 190b	3712 ± 157a
Ratio of DHCEOc	4		3.5		1

Regular FBS was used here; no DHCEO detected in any system in the absence of compound 1 or 2; statistical analyses were performed against corresponding HF-control.

^ap < 0.05.

^bp < 0.0005.

^cRatio of total DHCEO formed from triol 2 to that formed from epoxide 1.