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Colocalization between BODIPY 493/503 signals and lipids visualized by Raman spectroscopy in intestinal cells. (A) The Raman image visualizes lipids (red) and proteins (blue) by molecule-specific spectra (B). (C) BODIPY 493/503 fluorescence and Raman lipid signals show a strong overlap with similar signal intensities. Average lipid spectra of indicated positions in panel C are shown in panel D. Bar, 10 μm.
