TABLE 1.
TGA measurements and quantification of BODIPY 493/503 signals based on microscopy and COPAS flow cytometry of the eat-2, daf-7 and glp-1mutants
| TAG measurementa | BODIPY microscopyb | BODIPY-COPASc | |
|---|---|---|---|
| C. elegans strain (growth temp) | Mean ng of TAG/worm ± SEM,% of wild-type | Mean FU/worm ± SEM,% of wild-type | Mean FU/worm ± SEM,% of wild-type |
| eat-2 (ad465) | 22.34 ± 1.80 | 57.28 ± 3,28 | 72.16 ± 10,39 |
| daf-7 (e1372) | 197.30 ± 2.94 | 193.15 ± 1.45 | 196.50 ± 9.50 |
| glp-1 (e2141) (15°C) | 85.36 ± 4.33 | 100.30 ± 4.94 | 107.10 ± 5.99 |
| glp-1 (e2141) (25°C) | 127.50 ± 17.99 | 154.75 ± 30.15 | 150.85 ± 3.85 |
Biochemical, TAG, measurement was made for each homogenate in duplicates in two independent measurements, respectively. We performed two to three independent experiments for each genotype and condition with two replicates, respectively.
BODIPY 493/503 mean intensity (FU, fluorescence units) obtained by microscopy was measured for 20–30 worms from two to three independent experiments. Representative BODIPY 493/503 phenotypes are shown in Fig. 4.
BODIPY 493/503 mean intensity (FU, fluorescence units) obtained by COPAS flow cytometry was measured for 250–2,000 nematodes in each of two to three independent experiments. Data shown are means ± SEM of two to three independent experiments.