FIG.3.

Analysis of artificially mixed PCR products of selected uropathogens. Several equimolar mixtures of amplicon C (0933F/1407R) from selected uropathogens were analyzed with the analytical gradient mixed species at 62.0°C. Twenty microliters of each mixture was used for injection. The retention times are the time points when the DNA fragments are eluted from the column of the WAVE System and are indicated in minutes. The amount of DNA eluted is indicated as the absorbance (in millivolts). The experiments were done in triplicate. (A) mixture of the species E. faecalis and E. faecium; (B) E. faecalis and E. coli; (C) E. coli and P. aeruginosa; (D) mixture of the three species S. saprophyticus, K. pneumoniae, and S. agalactiae; (E) mixture of the four species S. saprophyticus, E. faecalis, K. pneumoniae, and E. coli. Panel F: mixture of the five species S. aureus, E. faecalis, K. pneumoniae, E. coli, and P. aeruginosa. The duration of one analysis was 16 min.