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Primers and their potential modifications for amplification of 16S rRNA
| 5′-3′ sequence | Primera | Reference |
|---|---|---|
| GCA CAA GCG GTG GAG CAT GTG G | 0933F | This study |
| GAC GGG CGG TGT GTA CAA G | 1407R | This study |
| CAG GCC TAA CAC ATG CAA GTC | 0063F | 9 |
| GGG CGG WGT GTA CAA GGC | 1387R | 9 |
| GCCCCCGCCG | GC(10F) | This study |
| CGCCCGCCGCGCCCCGCGCCCGTCCCGCCGCCCCCGCCCG | GC(40F) | 16 |
F, forward primer; R, reverse primer. 10F and 40F, GC clamp of 10 or 40 bp, respectively, as attachment at the 5′ end of forward primer 0933F.
