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. Author manuscript; available in PMC: 2011 May 9.
Published in final edited form as: Urol Oncol. 2009 JAN-FEB;27(1):53–63. doi: 10.1016/j.urolonc.2008.07.036

Figure 4. The effect of trastuzumab treatment on ER-a expression.

Figure 4

LTLTCa cells were transferred to IMEM without phenol red 24 hours before the beginning of the experiment. The next day, they were treated with the indicated agents for the specified amount of time. The cell lysates were prepared as described [75]. The effect of trastuzumab treatment at various time points on protein expression of p-ELK-1, p-MAPK, p-p90RSK and ER-a in LTLT-Ca cells is shown. Protein expression was examined using western immunoblotting. Blot shows phospho-Elk-,1 at 60 kDa, phospho-p90RSK at 90 kDa, p-MAPK at 42-44 kDa and ER-a at 66 kDa. The blots show a single representative of three independent experiments.