. Author manuscript; available in PMC: 2011 May 9.

Published in final edited form as: Nat Cell Biol. 2006 Nov 19;8(12):1407–1414. doi: 10.1038/ncb1506

Table 1.

Mutations in eIF4A augment Dpp signalling

Genotype	Viability (n)	Egg hatching rate (n)	Phenotype
Mad sax¹/+	100%	0% (209)	Eggs weakly ventralized
Mad sax¹/eIF4A¹⁰⁰⁶	100%	24% (195)	Eggs normal
Mad sax¹/eIF4A^R321H	100%	95% (187)	Eggs normal
tkv¹/tkv⁴	23% (230)	ND	Wing veins thickened
tkv¹/tkv⁴ eIF4A¹⁰⁰⁶	94% (304)	ND	Minor wing-vein defects
tkv¹/tkv⁴ eIF4A^R321H	101% (226)	ND	Wing veins normal
hs–Gal4/+;UAS–dpp/+ (plus heat-shock)	98% (203)	ND	Ectopic wing veins
hs–Gal4/+; eIF4A^R321H/+; UAS–dpp/+ (plus heat-shock)	0% (349)	ND	Lethality
hs–Gal4/+; UAS–eIF4A/+; UAS–dpp/+ (plus heat-shock)	100% (128)	ND	Wing veins normal

The viability of flies of a particular genotype was determined by comparing with siblings from the same cross from which they were derived. The egg hatching rate was calculated for eggs produced by viable females of the indicated genotypes that were mated with wild-type males. Percentage viability was calculated by comparing with siblings without inherited eIF4A mutations or transgenes from the same cross. n indicates the number of embryos or adult flies examined. ND, not determined. Heat-shock was administered by incubating pupae at 37 °C for 30 min at 18 h after puparium formation.