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. Author manuscript; available in PMC: 2011 Aug 9.
Published in final edited form as: Angew Chem Int Ed Engl. 2010 Aug 9;49(34):5970–5974. doi: 10.1002/anie.201002050

Figure 4.

Figure 4

Proteomic analysis of AOA-labeled MetRS-NLL S. typhimurium-infected mammalian cells. a) A total of 218 S. typhimurium proteins were identified from infected Raw264.7 cells, of which 185 (85%) were Salmonella proteins and 33 were mouse proteins. b) Comparison of Salmonella proteins identified from infected macrophages and Salmonella grown in minimal liquid culture. 376 proteins were identified in liquid culture only, 89 proteins were identified in infected macrophages only and 96 were identified in both samples. c) Comparison of proteins identified from AOA-pulse-labeled infected Raw264.7 cells (amino acid reporter) and a previously published dataset of Salmonella proteins identified from infected Raw246.7 cells under comparable conditions without AOA labeling (no amino acid reporter).[4] 100 proteins were exclusively identified in our data set, 85 proteins were identified in both datasets and 49 proteins were only identified in the previously published dataset.[4]