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. Author manuscript; available in PMC: 2011 May 9.
Published in final edited form as: Mech Dev. 2003 Dec;120(12):1455–1468. doi: 10.1016/j.mod.2003.09.004

Fig. 1.

Fig. 1

Specificity of the anti-phospho-STAT92E antibody. (A) STAT92E activation was detected using an anti-pSTAT92E antibody (brown) in the central and terminal regions of stage 5 wild-type embryos. (B) pSTAT92E signals resolve into 14 parasegmental stripes as well as head and gut regions in stage 9 wild-type embryos. (C) This pattern was absent in stage 5 stat92E mat− embryos, and (D) was greatly diminished in hop mat− embryos. All embryos are arranged anterior to the left and dorsal up. (E). Western blot of protein extracts from hop GOF, wild-type, and hop mat− embryos. Note the band corresponding to pSTAT92E has a higher intensity in the hop GOF lane than wild type and is much reduced in the hop mat− lane. (F). S2 cells treated with vanadate/H2O2 for 0 min (untreated) or 30 min to stimulate STAT92E signaling were stained with the anti-pSTAT92E and anti-STAT92E antibodies, respectively. Note increased staining by anti-pSTAT92E, but not regular STAT92E, antibody following 30 min vanadate/H2O2 treatment.