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Antigen release assay. (a) OD index of secreted GM in A. fumigatus and A. niger after 24 h of growth (means ± standard deviations [error bars] from three experiments). (b) Ratio of secreted GM relative to growth control is shown for one A. fumigatus isolate with a high MIC (Af33), one A. fumigatus isolate with a low MIC (Af27), and one A. niger isolate (An10). The drug concentration at which the ratio approximated 0.5 was interpreted as the MIC50.
