Table 1.
List of lung cancer biomarker candidates screened by label-free MALDI MS and their verification result on MRM.
| MALDI MS | MRM | ||||||
|---|---|---|---|---|---|---|---|
| Uniprot ID | Protein Name | Peptide Sequence | t-test † | Log2(LC/Control) | t-test ‡ | Log2(LC/Control) | Correlation Coefficient |
| P04217 | Alpha-1B-glycoprotein | CEGPIPDVTFELLREGETKAVK | - | 0.67 | - | - | - |
| FALVREDR | 3.8E-03 | -0.38 | 0.13 | 0.24 | 0.315 | ||
| P01011 | Alpha-1-antichymotrypsin | EQLSLLDRFTEDAK | 1.4E-03 | 0.80 | 0.077 | 0.33 | 0.106 |
| FTEDAKRLYGSEAFATDFQDSAAAK | 4.6E-03 | 0.58 | - | - | - | ||
| PQDTHQSR | 5.6E-03 | 0.84 | - | - | - | ||
| P43652 | Afamin | DGLKYHYLIR | 1.1E-03 | -0.93 | - | - | - |
| P02746 | Complement C1q subcomponent subunit B | GNLCVNLMR | 6.5E-03 | 0.87 | - | - | - |
| P00736 | Complement C1r subcomponent | CLPVCGKPVNPVEQR | - | 0.42 | - | - | - |
| DYFIATCK | 6.9E-03 | 0.49 | 0.15 | 0.14 | -0.238 | ||
| P00450 | Ceruloplasmin | YTVNQCR | 0.017 | 1.1 | 3.1E-04 | 0.29 | 0.411* |
| P10909 | Clusterin | YVNKEIQNAVNGVK | 0.01 | 0.33 | 0.19 | -0.87 | -0.015 |
| P06681 | Complement C2 | TAVDHIREILNINQK | 2.7E-03 | 1.4 | 0.051 | 0.23 | N/A |
| P01024 | Complement C3 | AGDFLEANYMNLQR | 5.9E-05 | -1.4 | 3.8E-04 | -1.3 | 0.690** |
| ILLQGTPVAQMTEDAVDAER | 2.1E-04 | -1.3 | 0.019 | -0.87 | 0.448* | ||
| KGYTQQLAFR | 2.8E-03 | -1.1 | 3.9E-05 | -1.3 | 0.800** | ||
| QPSSAFAAFVKR | 6.7E-03 | -1.3 | 2.0E-03 | 0.24 | -0.455 | ||
| WLNEQR | 2.5E-03 | -1.0 | 7.8E-06 | -1.5 | 0.521** | ||
| P01031 | Complement C5 | FWKDNLQHKDSSVPNTGTAR | 0.012 | 0.67 | - | - | - |
| TLRVVPEGVKR | 0.066 | 0.80 | - | - | - | ||
| P13671 | Complement component C6 | IEEADCKNKFR | 0.011 | 1.2 | - | - | - |
| P10643 | Complement component C7 | VFSGDGKDFYR | 9.5E-03 | 0.81 | - | - | - |
| P02748 | Complement Component C9 | FTPTETNKAEQCCEETASSISLHGK | 2.4E-04 | 1.4 | 6.1E-03 | 0.49 | 0.472* |
| QYTgTSYDPELTESSGSASHIDCR | 1.9E-03 | 1.1 | 3.8E-03 | 0.57 | 0.769** | ||
| P22792 | Carboxypeptidase N subunit 2 | SQCTYSNPEGTVVLACDQAQCR | 1.4E-03 | 0.56 | 0.062 | 0.16 | 0.372 |
| P00748 | Coagulation factor XII | CTHKGRPGPQPWCATTPNFDQDQR | 4.3E-03 | 1.2 | - | - | - |
| Q9UGM5 | Fetuin-B | MSPPQLALNPSALLSR | 3.0E-03 | 0.62 | 0.03 | 0.58 | 0.189 |
| P02751 | Fibronectin | AQITGYR | 1.1E-03 | -0.47 | 0.077 | -0.18 | 0.774** |
| GFNCESKPEAEETCFDKYTGNTYR | 1.9E-03 | -0.69 | 0.42 | -0.11 | 0.146 | ||
| IGFKLGVRPSQGGEAPR | 5.7E-03 | -0.90 | - | - | - | ||
| P26927 | Hepatocyte growth factor-like protein | RVDRLDQR | 6.1E-03 | -0.57 | 0.013 | 0.38 | -0.128 |
| P18065 | Insulin-like growth factor-binding protein 2 | LAACGPPPVAPPAAVAAVAGGAR | 2.5E-03 | 0.67 | - | - | - |
| Q06033 | Inter-alpha-trypsin inhibitor heavy chain H3 | EHLVQATPENLQEAR | 4.6E-03 | 0.97 | 3.6E-03 | 0.65 | 0.578** |
| Q14624 | Inter-alpha-trypsin inhibitor heavy chain H4 | EKNGIDIYSLTVDSR | 4.7E-04 | 0.65 | 0.062 | 0.40 | 0.368 |
| ETLFSVMPGLK | - | 0.09 | 0.01 | 0.42 | 0.331 | ||
| MNFRPGVLSSR | 9.8E-03 | 0.40 | 1.0E-03 | 0.63 | 0.413* | ||
| SPEQQETVLDGNLIIRYDVDR | 0.013 | 1.2 | - | - | - | ||
| P03952 | Plasma kallikrein | CQFFTYSLLPEDCKEEKCK | - | -0.32 | - | - | - |
| P01042 | Kininogen-1 | RPPGFSPF | 2.0E-04 | -2.0 | 5.1E-04 | -1.74 | 0.876** |
| P27918 | Properdin | TCNHPVPQHGGPFCAGDATR | 7.2E-04 | -0.52 | - | - | - |
| Q13103 | Secreted phosphoprotein 24 | DSGEDPATCAFQR | 3.7E-03 | -0.53 | - | - | - |
†: "-" indicates candidate screened by present/absent search,
‡: "-" indicates no MRM data obtained, *: P < 0.05, **: P < 0.01,
g: indicates site of O-linked glycosylation
N/A: valid value too small for calculation of correlation threshold.