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. 2011 May 9;6(5):e19867. doi: 10.1371/journal.pone.0019867

Figure 5. Affinity purification and purity analysis of cMAb CK3.

Figure 5

For purification and purity analysis of cMAb, the pooled cMAb CK3 supernatant samples (∼1 L) were purified by the ÄKTAprime plus using an affinity chromatography column (HiTrap rProtein A-FF). The pooled cMAb fractions were dialyzed and purification yield were evaluated by the modified human IgG1 ELISA. The purity of cMAb CK3 was analyzed by the SDS-10% PAGE.