Table 1. Three selected hybridoma clones to convert stable cMAbs against BP and BM.

cMAb ID	3 selected hybridomas converted into cMAbsa				Chimeric chain IDb
	Hybridoma clone ID	Antigen injected	Isotype	Cross-reactivity	Heavy(mVH+HcG1)	Light(mVL+HcK)
cMAb CK1	BP7 10B11	BP 430	IgG1/κ	BP/BM: +; BT: −	H1	L1
cMAb CK2	BP7 2C6	BP 430	IgG2a/κ	BP/BM: +; BT: −	H2	L2
cMAb CK3	BP1 7F7	BP 8324	IgG3/κ	BP/BT: +; BM: −	H3	L3

^aThese 3 hybridoma clones were selected by their binding specificities, antigen reactivities to their bacterial cell components, and in vitro/vivo neutralizing activities against B. pseudomallei (BP) 8324 and/or B. mallei (BM) ATCC 23344. BT: B. thailandensis.

^bEach chimeric chain was assembled by the overlapping PCR. mVH: mouse variable heavy chain; HcG1: human constant gamma 1; mVL: mouse variable light chain; HcK: human constant kappa chain.