TABLE 2.

The family of HPr-like proteins^a

Phylum	Organism	Gb	HPre	GI	Clusterc	HPrKd
Firmicutes
Bacilli	Bacillus subtilis	•	PTSH	16078454	IIABC-H-I	•
	Bacillus anthracis	•	BA4727	21402102	IIABC-H-I	•
	Bacillus megaterium		PTHP	13633375
	Bacillus halodurans	•	PTSH	15615636	H-I	•
	Geobacillus stearothermophilus		PTHP	1172717	H-I
	Staphylococcus carnosus		PTHP	131538
	Staphylococcus xylosus		PTSH	11596363		•
	Staphylococcus aureus	•	PTSH	15924073	H-I	•
	Lactobacillus casei		PTHP	13633570		•
	Lactobacillus sakei		PTHP	3122641
	Lactobacillus gasseri		LGASP108	23003237	H-I	•
	Lactobacillus brevis		PTSH	14133565		•
	Leuconostoc mesenteroides		LMESP115	23024451	H-I	•
	Lactococcus lactis	•	PTHP	13633652	H-I	•
	Enterococcus faecalis		PTHP	1172718		•
	Streptococcus mutans		PTHP	1172720		•
	Streptococcus salivarius		PTHP	1172721		•
	Streptococcus bovis		PTHP	13633635		•
	Streptococcus pyogenes	•	PTSH	15675305	H-I	•
	Streptococcus pneumoniae	•	SP1177	15901042	H-I	•
	Streptococcus thermophilus		PTSH	17978653
	Streptococcus agalactiae	•	PTSH	22536985	H-I	•
	Oceanobacillus iheyensis	•	OB2344	23099799	H	•
	Oenococcus oeni		OOENP127	23038069		•
	Listeria innocua	•	PTSH	16800070	H-I	•
	Bacillus subtilis	•	CRH	16080527	j-k-l-H	•
	Bacillus halodurans	•	CHR	15616128	j-k-l-H	•
	Bacillus anthracis	•	BA0239	21397614	j-k-l-H	•
	Oceanobacillus iheyensis	•	OB2465	23099920	j-k-l-H	•
Clostridia	Thermoanaerobacter tengcongensis	•	FRUB	20808237	j-k-x10-l-H	•
	Clostridium thermocellum		CHTEP203	23020129	j-k-x-l-H	•
	Thermoanaerobacter tengcongensis	•	TTE0115	20806644	H	•
	Clostridium thermocellum		CHTEP180	23020106	H	•
	Clostridium thermocellum		CHTEP182	23021750	H-I	•
	Clostridium acetobutylicum	•	CAC1820	15895096	H	•
	Clostridium perfringens	•	HPR	18310651	H	•
Mollicutes	Mycoplasma capricolum		PTHP	1172719
	Mycoplasma genitalium	•	PTSH	12044891	H	•
	Mycoplasma pneumoniae	•	PTSH	13507792	H	•
	Mycoplasma pulmonis	•	MYPU6030	15829074	H-I	•
	Ureaplasma urealyticum	•	PTSH	13358152	H	•
Proteobacteria
α subdivision	Mesorhizobium loti	•	MSL5292	13474414	H-I	•
	Mesorhizobium loti	•	MSL5090	13474245	K′-IIA-H	▪
	Sinorhizobium meliloti	•	SMC02754	15963793	K′-IIA-H	▪
	Agrobacterium tumefaciens	•	AGRC49	15887388	K′-IIA-H	▪
	Brucella melitensis	•	BMEI2031	17988314	K′-?-IIA-H	▪
	Rhodopseudomonas palustris		RPALP163	22962348	K′-IIA-H	▪
	Caulobacter crescentus	•	CC0241	16124496	K′-IIA-H	▪
	Rhodobacter sphaeroides		RSPHP157	22957984	K′-j-IIA-H	▪
	Magnetospirillum magnetotacticum		MAGNP479	23011831		▪
	Novosphingobium aromaticivorans		SAROP34	23110205	K′-j-IIA-H	▪
	Rhodospirillum rubrum		RRUBP475	22965880	K′-j-IIA-H-I	▪
	Magnetospirillum magnetotacticum		MAGNP76	23015045	K′-j-IIA-H-I	▪
β subdivision	Ralstonia eutropha		PTHP	131532
	Ralstonia solanacearum	•	PSTH	17545066	IIA-H-I	•
	Ralstonia metallidurans		REUTP495f	22980242	IIA-H-I	•
	Nitrosomonas europaea		NEURP196	22955983	-	•
	Burkholderia fungorum		BCEPP510	22987169	IIA-H-I	•
	Neisseria meningitidis	•	NMB2045	15677867	IIA-H-I	•
γ subdivision	Pseudomonas putida		PTHP	13633649
	Pseudomonas aeruginosa	•	PA4466	15599662	N-h-IIA-j-H
	Pseudomonas fluorescens		PFLUP387	23061779	N-h-IIA-j-H
	Pseudomonas stutzeri		PTSO	22138783
	Microbulbifer degradans		MDEGP161	23027798	N-IIA-j-H
	Azotobacter vinelandii		AVINP175	23103593	N-h-IIA-j-H
	Escherichia coli	•	PTSH	15802948	H-I-IIA	/PICK>
	Klebsiella pneumoniae		PTHP	131536
	Yersinia pestis	•	PTSH	16123174	H-I-IIA
	Serratia marcescens		PTSH	21039016
	Haemophilus influenzae	•	HI1713	16273600	H-I-IIA
	Haemophilus somnus		HSOM0557	23466976	H-I-IIA
	Pasteurella multocida	•	PTSH	15602763	H-I-IIA
	Buchnera sp.	•	PTSH	15616690	H-I-IIA
	Buchnera aphidicola	•	PTSH	21622959	H-I-IIA
	Vibrio cholerae	•	VC0966	15640982	H-I-IIA
	Salmonella enterica	•	PTSH	16765751	H-I-IIA
	Escherichia coli	•	PTSO	15803746	N-h-IIA-j-H
	Klebsiella pneumoniae		PTSO	1709908
	Salmonella enterica	•	PTSO	16762086	N-h-IIA-j-H
	Yersinia pestis	•	PTSO	16123729	N-h-IIA-j-H
	Proteus mirabilis		PTSO	13633651
	Shewanella violacea		PTSO	13633605
	Vibrio cholerae	•	VC2533	15642528	N-h-IIA-j-H
	Escherichia coli	•	Z4879	15804017	IIA-IIB-IIC-sk-H
	Salmonella enterica	•	STY4004	16762540	IIB-IIC-sk-x-H
	Xanthomonas campestris	•	PTSH	21232239	N-h-IIA-K-j-IIA-H-I	•
	Xanthomonas axonopodis	•	PTSH	21243705	N-h-IIA-K-j-IIA-H-I	•
	Xylella fastidiosa	•	XF1403	15838004	N-h-K-j-IIA-H-I	•
δ subdivision	Geobacter metallireducens		GMETP32	23056421	N-h-K-j-IIA-H-I	•
	Desulfovibrio desulfuricans		DDES2010	23475093	IID-H-I
Fusobacteria	Fusobacterium nucleatum	•	FN1782	19705087	H	•
	Fusobacterium nucleatum	•	FN1794	19705099	H-I	•
Actinobacteria	Corynebacterium glutamicum	•	CGL1892	19553142	IIABC-H
	Streptomyces coelicolor	•	PTSH	21224185	H
	Bifidobacterium longum	•	PTSH	23465010	H-I
Chlamydiae	Chlamydia trachomatis	•	PTSH	15605060	H-I
	Chlamydia muridarum	•	TC0614	15835231	H-I
	Chlamydophila pneumoniae	•	PTSH	15617961	H-I
Spirochaetes	Borrelia garinii		AAG28175	11055615
	Borrelia burgdorferi	•	BB0557	15594902	H-I-IIA
	Borrelia burgdorferi	•	BB0448	15594793	N-h-H
	Treponema pallidum	•	TP0589	15639577	K-x-H	•
Chlorobi	Chlorobium tepidum	•	PTSH	21648255	H	•
Chloroflexi	Chloroflexus aurantiacus		CHLOP311	22973407

^athe sequences were extracted from the NCBI protein database by a BLAST search using the B. subtilis ptsH sequence gene initially as the query. All unique HPr sequences in the database were reported together with the unique GI number of the entry in the database.

^bA bullet indicates that the whole genome sequence of an organism is available.

^cClustering of genes, possibly in an operon-like structure, on the genome. H, HPr-like protein; N, σ^N transcription factor; I, PTS enzyme I; IIABC, PTS enzyme IIABC; IIA, IIB, IIC, IID, PTS enzymes IIA, IIB, IIC, and IID; K and K′, normal and short version of HPrK/P homologue, respectively; j, h unknown conserved genes; sk, putative sugar kinase.

^dA dot or square indicates the presence in the organism of a normal or short version of an HPrK/P homologue, respectively (taken from Table 1).

^eTypical and similar relationships. A typical sequence (bold) represents a group of similar sequences (indented) with at least 55% sequence identity.

^fREUTP495 is 140 residues long, which most probably is an annotation error. The true translational start is likely to correspond to the Met residue at position 52.