TABLE 1.
Eukaryotic organisms exhibiting RNAi-related phenomena
| Kingdom | Species | Stage tested | Delivery method | Reference(s) |
|---|---|---|---|---|
| Protozoans | Trypanosoma brucei | Procyclic forms | Transfection | 52 |
| Plasmodium falciparum | Blood stage | Electroporation and soaking | 143, 150 | |
| Toxoplasma gondii | Mature forms in fibroblast | Transfection | 4 | |
| Paramecium | Mature form | Transfection and feeding | 14 | |
| Leishmania donovanii | Tried but not working | 183 | ||
| Invertebrates | Caenorhabditis elegans | Larval stage and adult stage | Transfection, feeding bacteria carrying dsRNA, soaking | 26, 31 |
| Caenorhabditis briggsae | Adult | Injection | 79 | |
| Brugia malayi (filarial worm) | Adult worm | Soaking | 1 | |
| Schistosoma mansoni | Sporocysts | Soaking | 23 | |
| Hydra | Adult | Delivered by micropipette | 49 | |
| Planaria | Adult | Soaking | 49 | |
| Lymnea stagnalis (snail) | Adult | Injection | 122 | |
| Drosophila melanogaster | Cell lines, adult, embryo | Injection for adult and embryonic stages, soaking and transfection for cell lines | 96, 114, 155 | |
| Cyclorrphan (fly) | Early embryonic stages | Injection | 200 | |
| Milkweed bug | Early embryonic stages | Injection | 102 | |
| Beetle | Early embryonic stages | Injection | 27 | |
| Cockroach | Larval stage | Injection | 146 | |
| Spodoptera frugiperda | Adult and cell line | Injection and soaking | 176, 215 | |
| Vertebrates | Zebra fish | Embryo | Microinjection | 224 |
| Xenopus laevis | Embryo | Injection | 162 | |
| Mice | Prenatal, embryonic stages, and adult | Injection | 31, 229 | |
| Humans | Human cell lines | Transfection | 42 | |
| Plants | Monocots/dicots | Plant | Particle bombardment with siRNA/transgenics | 88 |
| Fungi | Neurospora crassa | Filamentous fungi | Transfection | 51 |
| Schizosaccharomyces pombe | Filamentous fungi | Transgene | 178 | |
| Dictyostelium discoideum | Transgene | 147 | ||
| Algae | Chlamydomonas reinhardtii | Transfection | 231 |