Figure 4. Tyrosine phosphorylation detected by anti-pY Western blotting of proteins in neuron lysates and pY IPs after NT-3 treatment.
Neurons were treated with or without NT-3 as indicated in the Materials and Methods. Total cell lysates and pY IPs were probed with PY99-HRP. Numbers to the left of the gel show MW × 10−3 corresponding to protein MW standards.