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. Author manuscript; available in PMC: 2012 May 15.
Published in final edited form as: J Neurosci Methods. 2011 Mar 23;198(1):70–76. doi: 10.1016/j.jneumeth.2011.03.013

Fig. 5. Internal architecture of Nrl−/− photoreceptors.

Fig. 5

Shown are (A) Photoreceptors from a critical point dried sample of Nrl−/− mouse retina and (B) subsequent deposition of platinum on top of a region of interest to protect the surface from milling artifacts during sectioning. (C) A created trench allows visualization of the internal architecture of Nrl−/−photoreceptors with (D) a zoomed image of a single Nrl−/−photoreceptor used for reconstruction shown. (E) The resulting three-dimensional reconstruction of an Nrl−/−photoreceptor and its disrupted internal disc elements. Towards the inner segment, the disc elements stack normally and retain some connection to the outer segment. The Nrl−/−photoreceptor inner segment is shown in gray and the outer segment in transparent gray. Disc elements inside the outer segment are shown in blue and green with the disrupted elements in red. Scale bars in panels A, B, C and D are 10, 10, 10, and 1 µm, respectively.