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Ca2+ imaging of ventral spinal neurons in KCl-treated embryos. The incidence of calcium spiking in presumptive spinal cords dissected at stage 15 and treated with 25 mm KCl is lower than the incidence in controls. The frequency of calcium spiking in these KCl-treated spinal cords is also less than the frequency in controls (**p < 0.01). Values are means ± SEM for n = 6–8 embryos/condition.
