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. Author manuscript; available in PMC: 2012 May 10.
Published in final edited form as: Curr Biol. 2011 Apr 21;21(9):756–761. doi: 10.1016/j.cub.2011.02.037

Figure 3.

Figure 3

NMO acts to stabilize CLK. Flies were entrained for three days under LD conditions and collected at the indicated times. (A) Western blot of head extracts from ClkJrk, w1118;+/UAS-nmoRNAi-2 (RNAi control) and w1118;UAS-nmoRNAi-2/tim-Gal4;+/UAS-Dcr-2 (nmoRNAi) flies probed with CLK, PER and TIM antisera. Bands corresponding to the different phosphorylation states of CLK, PER and TIM are marked as in Fig. 2. Actin serves as a loading control. (B) Western blot of head extracts from ClkJrk, w1118;UAS-GFP-nmo/+ (OX control) and w1118;UAS-GFP-nmo/tim-Gal4 (nmoOX) flies probed with CLK, PER and TIM antisera. Bands corresponding to the different phosphorylation states of CLK, PER and TIM are marked as above. Actin serves as a loading control. See also Fig. S3.