Figure 6. eIPSCs recorded in the Im.

A, scheme of the amygdala depicting the location of the stimulating and recording electrodes. B, voltage clamp recording showing IPSCs evoked by Im stimulation at increasing intensities. The average of 3 traces for each intensity (range, 5–50 μA, step of 5 μA) is shown in grey. An eIPSC after 15 μA stimulation is illustrated in black. C, the input–output curve shows the relation between the intensity of stimulation used and the peak amplitude of the eIPSCs. Each point of the graph represents the average value of 3 sweeps. Note the presence of failures elicited by lower stimulation intensities. The arrow indicates response evoked by ‘minimal’ stimulation intensity. D, voltage clamp recording showing paired-pulse facilitation of IPSC evoked by Im stimulation. Individual eIPSCs are shown in grey; the average of 10 traces is shown in black. All eIPSCs were recorded in the presence of kynurenic acid (3 mm), and were mediated by GABA_A receptors, since they were abolished by bicuculline (5 μm; not shown). Abbreviations: D, dorsal; L, lateral; M, medial; V, ventral; BLA, basolateral complex; CE, central nucleus; Im, main intercalated nucleus; Rec., recording electrode, Stim., stimulating electrode.