Figure 4.

Immunofluorescent staining. (a) A LnCAP cell spiked into blood and captured on the microchannel, stained for CK (green) and also nuclear stained with DAPI (blue). A small WBC is seen with only the nucleus stained blue. (b) A cluster of CTCs from a clinical lung cancer sample captured on the microchannel that are stained for CK. These cells were CD45-negative and DAPI-positive (not shown). (c) A cluster of cells from lung cancer showing triple staining with CK (green), CD45 (red), and DAPI (blue). Three CK-positive CTCs are shown with 2 smaller WBCs stained positive for CD45.