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Costaining clinical lung cancer CTCs with anti-CK and CEE-Enhanced. (a–c) A single CTC on the microchannel stained with anti-CK (a), CE-AlexaFluor-546 (orange, (b)), and (c), a composite image. (d–f) shows the same order of staining but with 2 attached CTCs. This demonstrates the costaining of the internal CK antigen and the cell surface antigens with CE.
