Figure 2.

Differentiation of primary (A2B5⁺) OPC cultures into mature O1⁺ oligodendrocytes can be increased or decreased by adding or blocking TIMP-1, respectively. A, Following a 48 h treatment with rmTIMP-1 (10 ng/ml) in purified immunopanned A2B5⁺ rat OPC cultures, increased numbers of mature (O1⁺) oligodendrocytes were observed. Control cultures were also treated for 48 h in similar volumes, but lacking TIMP-1. ***p < 0.001, t test. B, Administration of a blocking anti-TIMP-1 antibody (0.6 μg/ml) to immunopanned A2B5⁺ cultures for 48 h decreased O1⁺ cell numbers compared with control IgG (1 μg/ml). **p < 0.0006, t test. C, All data using A2B5⁺ cultures are representative of three independent experiments performed in triplicate using coded samples in a double-blinded fashion. Error bars represent the mean ± SEM.