. Author manuscript; available in PMC: 2012 May 1.

Published in final edited form as: Anal Chem. 2011 Mar 30;83(9):3548–3554. doi: 10.1021/ac2002565

Table 1.

Optimization and screening of matrix.^a

Entry	Additive	Incubation Time (min)	Matrix	Signal m/z (% relative abundance)
1	none	-	CHCA	1304.9 (100), 1342.8 (74.4), 2200.1 (28.9)
2	0.1 M pinacol	5	CHCA	1025.9 (21.4), 1028.9 (24.0), 1174.0 (27.4), 1304.8 (77.7), 1342.8 (100)
3	0.1 M pinacol	30	CHCA	1304.9 (97.6), 1342.9 (100)
4	0.1 M pinacol	30	HPA	No Signal
5	0.1 M pinacol	30	DABP	1304.8 (100), 1736.2 (86.3),^b 2093.3 (48.8)
6	0.1 M pinacol	30	CMBT	1304.7 (100), 1736.0 (84.1)^b
7	0.1 M pinacol	30	CHIQ	2126.2 (100)^c
8	0.1 M pinacol	30	DHAP	1304.8 (27.0), 2126.2 (100)^c
9 ^a	0.1 M pinacol	30	DHB	1304.7 (100), 2163.0 (79.2)^d

Sample utilized was FLBP1 [FITC-(F_BPARW)(FRW)*VRD]; F_BPA = 4-borono-L-phenylalanine;

branching lysine. All samples were subjected to a 10 second on plate water wash before analysis. Signals < 20% abundance not reported.

[(M+H)⁺ - FITC].

(M+H)⁺.

(M+DHB+H)⁺