. Author manuscript; available in PMC: 2012 May 1.

Published in final edited form as: Anal Chem. 2011 Mar 30;83(9):3548–3554. doi: 10.1021/ac2002565

Table 2.

Transesterification of 1,2-diols with DHB.^a

Entry	Diol	MALDI Matrix	Predicted Peak ([(M+diol+H)⁺- FITC]/(M+diol+H)⁺)	Signal m/z (% relative abundance)
1	Ethylene glycol	CHIQ	1680.0/2069.0	1304.4 (100), 1680.0 (28.0), 2068.7 (42.5)
2	Ethylene glycol	DHB	1771.9/2161.0	1304.6 (100), 1771.8 (48.0), 1813.7 (20.4), 2161.8 (99.2)
3	Glycerol	CHIQ	1710.0/2099.0	1304.7 (100), 1710.0 (42.3), 2099.0 (58.0)
4	Glycerol	DHB	1771.9/2161.0	1304.7 (100), 1772.0 (88.8), 2162.0 (89.7)
5	Pinacol	CHIQ	1736.0/2125.1	1304.8 (85.9), 1736.0 (100), 2093.1 (33.0), 2125.1 (45.6)
6	Pinacol	DHB	1771.9/2161.0	1304.1 (68.7), 1771.8 (45.2), 2161.9 (100)
7	-	DHB	1771.9/2161.0	1304.4 (49.9), 1771.6 (34.8), 2162.5 (100)

Sample utilized was FLBP1. All diols were prepared as 0.1 M solutions, mixed with FLBP1 in a 1:1 (v:v) ratio, and allowed to incubate for 30 min. All samples were subjected to a 10 second on plate water wash before analysis. Signals < 20% abundance not reported.