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. Author manuscript; available in PMC: 2011 May 10.
Published in final edited form as: Nat Med. 2010 Jul 8;16(8):903–908. doi: 10.1038/nm.2187

Figure 3.

Figure 3

Ectopic MSI2 cooperates with BCR-ABL1 in promoting leukemia progression. (a) Immunoblot of BaF3 cells transduced with control vector MSCV-IRES-EGFP (MIG) or vector carrying MSI2 (M). (b) Numbers of EGFP+ BaF3 cells remaining 4 d after cytokine withdrawal. Representative of five independent experiments from two independent clones, details in Supplementary Methods. (c–f) Analysis of disease parameters including spleen and liver weight (c), white blood cell count (d) and bone marrow and spleen cellularity (e,f) in diseased mice killed at 14 d (representative of ten mice per group and two independent assays). (g) Flow cytometric analysis of spleen cells from leukemic mice, stained for Mac1 and Gr1 and gated on BCR-ABL1+GFP+ cells. Average percentages for the diseased cohort are indicated near gates for Mac1highGr1high and Mac1highGr1low populations, +P < 0.05, **P < 0.001. Supplementary Figure 10c contains statistical analyses. (h) H&E staining of bone marrow (BM) and spleen (SP) from diseased mice. Scale bars, 250 μm.