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. Author manuscript; available in PMC: 2012 Apr 19.
Published in final edited form as: Biochemistry. 2011 Mar 29;50(15):3181–3192. doi: 10.1021/bi1019622

Figure 11. CCG 50014 inhibits the Gαo dependent membrane localization of RGS4.

Figure 11

A) When overexpressed in HEK293T cells, GFP-RGS4 is localized to the cytosol. B) Co-expression with Gαo induces a subcellular translocation of the GFP-RGS4 to the plasma membrane. C) This translocation does not occur in response to co-expression with the RGS-insensitive Gαo mutant (G184S). D/E) Cells expressing Gαo and GFP-RGS4 show no change in the plasma membrane localization of the RGS when treated with vehicle (0.1% DMSO), however treatment with F/G) CCG-50014 (100μM) rapidly induces a loss of the plasma membrane localization of the RGS without diminishing the overall signal. Representative line scans across a cell treated with H) vehicle and I) CCG-50014 also show this effect. J) Quantification of this effect from 10 cells treated with vehicle or CCG-50014 (100 μM) shows a significant decrease in the amount of RGS-GFP located at the membrane after compound addition. A trend towards increased cytosolic localization after compound treatment is also observed, suggesting that the treatment doesn't just diminish the GFP signal. ****p<0.0001, *p<0.05.