Extracellular flux analysis of cardiomyocytes in medium containing pyruvate and/or glucose: (A) Bioenergetic function assay in cardiomyocytes having glucose (Glc only), pyruvate (Pyr only), or glucose and pyruvate (Glc + Pyr) as substrate. ATP-linked OCR (B), proton leak (C), non-mitochondrial OCR (D), reserve capacity (E), and the mitochondrial respiratory stateapparent (F) were calculated from the assay in panel A. n = 5 per group, *p<0.05 vs. Glc only group; #p<0.05 vs Pyr only group.